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KMID : 0914820130130030157
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Journal of the Korean Gastric Cancer Association
2013 Volume.13 No. 3 p.157 ~ p.163
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Disappearance of Serum Methylated p16 Indicates Longer Survival in Patients with Gastric Cancer
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Lim Han-Ki
Park Joong-Min
Chi Kyong-Choun
Lee Eun-Ju
Jeong Eun-Mi
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Abstract
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Purpose: The aim of this study was to assess clinical correlations with postoperative alteration of p16 DNA methylation, and to clarify whether postoperative changes in the serum DNA methylation status of p16 could be used as a reliable prognostic factor for gastric can-cer. Materials and Methods: Fifty-three consecutive gastric adenocarcinoma patients who underwent gastric resection (Chung-Ang Univer-sity Hospital, Seoul, Korea) were included. DNA methylation of p16 was evaluated by methylation-specific polymerase chain reaction using serum DNA preoperatively and at the 10th postoperative day. The correlation between changes in methylation status and patients’ prognosis was analyzed. Results: p16 was methylated in 79.2% of preoperative serum DNA and in 54.7% of postoperative serum DNA, respectively. Methyla-tion in p16 disappeared more frequently in patients who underwent standard D2 lymphadenectomy compared to those who underwent modified D1+ lymphadenectomy (P=0.016). Whereas methylation of preoperative serum DNA was not correlated with survival, pa-tients with postoperative disappearance of p16 methylation showed longer survival than those without postoperative disappearance of p16 methylation in the patients who had gastric cancer with lymph node metastasis (P=0.042). Conclusions: Postoperative disappearance of p16 methylation could be an available prognostic factor for node-positive gastric cancer.
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KEYWORD
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Stomach neoplasms, Methylation, Genes, p16
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